- Iron (Fe) is essential for all biota and plays main role in various processes of photosynthesis, DNA synthesis and respiration. Iron (Fe) deficiency is a common metabolic deficiency worldwide, affecting an estimated 3.7 million individuals worldwide. Presence of Fe in food plantations is a major worldwide issue because of limited iron in routine intake food. Mostly, Fe is deficient in Pakistani soil and causes significant decrease in yield. This deficiency can be overcome through various regulatory mechanisms in plants that will fulfill the requirements for animals and human beings indirectly. Biofortification is one of the most effective technique to tackle the deficiency of Fe. In biofortifying various types of bacteria, fungi and other useful microorganisms are applied to soil for increasing nutrients uptake especialy iron. These rhizosperic inocula release siderophores which are responsible for nutrients releasing from the soil surfaces. Biofortification have various types like agronomic biofortification, genetically fortification and through microbes. Among these use of microbes is an effective and newly evolved technology that can eliminates diseases like anemia in pregnant womens and school going children indirectly through plants. This review study explained that Biofortification looks to be a viable method for underfed people in remote rural locations, bringing naturally fortified food items to individuals who lack access to commercially marketed goods, which are more widely available in urban areas.

Keywords : Iron deficiency, Anemia, Biofortification, Siderophores, Microbes.