The waste cooking oil (WCO) is a problematic once its use is expired as it contributes to the environmental pollution especially to pollute our source of drinking water such as river, lakes and seas. Reusing the domestic waste such as cooking oil as an alternate substrate may be an economic solution to this problem. Bio-surfactant is one of among the potential product that distributed many advantages to the industries such as low toxicity, biodegradability and eco-friendly product. The study is done to investigate the potential bio-surfactant which produced from waste cooking oil and then compare it to the bio-surfactant produced from pure cooking oil (PCO). The parameter which has been analyzed are formability, pH, oil emulsification and hardness of water. The biosurfactant from WCO shows a positive result from all analysis and can be a potential alternative material for bio-surfactant production.

Keywords : Biosurfactant, Waste Cooking Oil, Pure Coconut Oil, Formability, pH, Oil Emulsification, Hard Water.