The chemical constituents of essential oil from air-dried leaves of Salvia officinalis L. growing in Nepal was determined by gas chromatography couple with mass spectrometry (GC/MS) technique. A total number of twenty-one phytoconstituents were identified representing 99.95% of total oil composition. The major chemical components were camphor (65.18%), camphane (9.73%), eucalyptol or 1,8-cineole (4.72%), 2-nepthalenementhanol (3.85%) and α-pienene (2.33%) whereas the minor chemical components were boreneol (1.45%), eucarvone (1.44%), benezenemethanol (1.36%), viridiflorol (1.23%), o-cymene (1.22%), limonene (1.07%), trans-pinocarveol (0.90%), 2-(3-oxobutyl)-cyclohexonone(0.84%), βcaryophyllene oxide (0.78%), β-pienene (0.78%), mcymene (0.72%), β-Ocimene (0.71%), 8-hydroxy-p-cymene (0.67%), bornylacetate (0.49%), valerenal (0.40%) and tricylene (0.11%). Since our S. officinalis lacks thujone content that poses higher risk of toxicity at high doses, it becomes more favorable to use for the treatment of several diseases in human. On the other hand, bacterial inhibitory activity of essential oil and the three different extracts was determined to evaluate its antibiotic potential using agar well disc diffusion method. The essential oil of S. officinalis L. leaves revealed strong antimicrobial potential against both Gram-positive Staphylococcus aureus, Gram-negative Klebsiella pueunmoniae and Escherichia coli bacteria. Among three extracts, the ether extract of S. officinalis L. leaves showed strong antimicrobial activity against Klebsiella pueunmoniae and Escherichia coli but weak against Staphylococcus aureus.

Keywords : Salvia Officinalis, Camphor, Camphene, Antimicrobial, Ether, Essential Oil