The expression or repression of a wide variety of genes, which code for protectins, controls vital physiological processes like metabolism, development and immune responses. The transcription of genes started by activation of the glucocorticoid in both negative and positive manners like metabolism, immunization, and inflammation. The transcription process is complex and involves a large number of factors interconnected by a large number of co-factor factors.In response to stress TTC5 is an active factor because interaction and stabilization and regulation of transcriptional activity of GR depends on this co factor. There are many other factors are also present but TTC5 is the main co factor because regulation of GR in gene depends on it. The TTC5 control of GR is expected to contribute to glucose corticus and GR's physiological function. The TTC5 regulates the transcription of GR target genes, including inflammation, involved in various processes. Duo TC5 has novel potential targets for different compounds which enable better control over glucocorticoid-related therapies, considering the importance of glucocorticoids in treating inflammatory disorders and in general clinical practice. Wild type (2kb) is present on the agarose gel (but it is very blurry). Mutant type (2kb) is present on the agarose gel. PET28a (5kb) is present on the agarose gel