This study was aimed for the development of callus and multiple shoots from germinating seeds and inter-nodal buds of Solanum xanthocarpum. The experimental result shows a good response of this plant to ½ Murashige and Skoog composition than to full strength medium. The medium was supplemented with different concentrations of 6- Benzyl amino purine, Indol Acetic Acid and naphthalene acetic acid separately and in combination. By increasing the concentration of growth regulator auxin Indol Acetic Acid and naphthalene acetic acid with a high concentration of cytokine 6- Benzyl amino purine resulted in the formation of callus alone which was whitish or brownish friable callus. The addition of 6- Benzyl amino purine alone in low concentration leads to the initiation of green compact callus. Shoot formation can be commenced by reducing the concentration of BAP and its optimum was observed as 0.5 mg/L, without any other growth regulators. Germinated seeds also observed to be very efficient in the production of green compact callus at this concentration. These calluses were cut and placed in a medium with the same concentration to induce shoot formation. The maximum number of multiple shoots (9-11 plantlets) was callus formed from seeds, than nodal explant resulting callus. Regenerated shoots were subjected to initiation of rooting in MS medium without growth regulators and successfully hardened and transferred to the greenhouse and later to the garden.

Keywords : Solanum xanthocarpum, Micropropagation, Regeneration, Callus, Shoot Formation.