The aim of this study was to assess the influences of heat reflective roof coating on indoor microclimate of building and blood serum biochemical parameters. Half of the building roof was coated with heat reflective coatings to investigate microclimate compared to non-coated roof for eight consecutive days. Collected blood samples from animals in both microclimate were divided into, control; under not- coated roof (n = 253) and treatment; under coated roof (n = 292) groups. The significant differences (P < 0.05) obtained for each parameters in both microclimate. The temperature and THI were significantly (P < 0.05) lower in treatment compared to control indoor environments, wherase the relative humidity was significantly (P < 0.05) higher in treatment indoor microcliamte. Studied blood parameters were not affected by indoor microclimate except for total colesterol and calcium which obtained significantly (P < 0.05) higher in treated indoor microclimate. These results demonstrated that heat reflective coating decrease heat invasion to inside the shelter and make effective way to modify microenvironment, and more attempts are needed to investigate blood parameters which were not affected by indoor microclimate variations.