This research study was designed to carry out biolelectrochemical remediation of petroleum contaminated wetland sediment obtained from Ekerekana-Ama Creek, Okrika, in Rivers State. Sediment Microbial Fuel Cells (SMFCs) consisting of an anode embedded in the anaerobic sediment containing petroleum hydrocarbon contaminants and a cathode suspended in the overlying aerobic water were used. Seven 300 ml sterile transparent bottles labelled T1 to T7 subjected to different treatments were used for bioelectrochemical remediation study. Gas Chromatography Mass Spectrophotometric analysis was done to check the level of reduction in petroleum hydrocarbon contaminants at the beginning (0 day) and end (80 days) of bioremediation. Percentage reduction in total petroleum hydrocarbon contaminant (272.63 ppm) in the sediment were 22.42 %, 65.80 %, 6.12 %, 21.70 %, 52.4 %, 27.70 %, 19.93 % for T1, T2, T3, T4, T5, T6 and T7 respectively. Distinct bacteria isolated were characterized biochemically and molecularly. The identity of the bacteria nucleotide sequences ( genus to species) as shown by Basic Local Alignment Search Tool (BLAST) identified Clostridium sporogenes (MF623797), Desulfobulbus propionicus (MF623798), Ewingella Americana (MF623799), Bacillus amyloliquefaciens (MF623800), Helicobacter sp. (MF623801), Alcaligenes faecalis (MF623802), Clostridium botulinum, Bacillus subtilis (MF623803), Klebsiella oxytoca (MF623804), and Burkholderia cepacia (MF623805) associated with T1 to T7. These isolated microorganisms interplayed metabolically to bring about remediation of petroleum hydrocarbon using a microbial fuel system.