Eucalyptus species, commonly referred to as Eucalyptus, are natives to Australia and the islands of north Eucalyptus is grown as an exotic plant species in tropical and subtropical regions of Africa, South America and Asia. The Present study was to analyze the effect of Indole 3 Acetic Acid on contamination and shoot elongation of Eucalyptus Camaldulensis in different concentration of MS media, to estimate the biochemical components in the leaves of Benzyl Amino Purine induced Eucalyptus Camaldulensis, to estimate the biochemical components in the shoot of Indole 3 Acetic Acid induced Eucalyptus Camaldulensis. The Eucalyptus Camaldulensis have potential biochemical metabolites in their leaves and shoot and has been proven to be effective for various purposes. Hence, the present study has been made to analyze the impact of Benzyl Amino Purine and Indole Acetic Acid on growth and shoot elongation of Eucalyptus Camaldulensis in various MS Media Concentration.