Phaius tankervilleae (Banks) Blume is one of the most widely valued ornamental orchids of the world. It is collected unsustainably for trade and consumption, the species has been decreased fast in natural habitats. Seeds of Phaius Tankervilleae were inoculated on Murashige and Skoog (MS) medium with various concentrations of auxin and cytokinin either alone or in combination. The germination and multiplication of seeds was successfully established in 30-45 days in MS medium fortified with 3% sucrose,0.8% agar supplemented with 2.5mg/L Benzyl Amino Purine (BAP) combination with 0.5 mg/L Naphthalene Acetic Acid(NAA). Subculturing on MS medium with 2.0 mg/l Benzyl Amino Purine (BAP) alone favored production of maximum number of shoots. Elongated shoots were separated and rooted on ½ strength MS medium with 1.5 mg/L Indole Butric Acid (IBA). Plantlets, thus developed were established in soil with 65% survivability

Keywords : In Vitro Propagation, Phaius Tankervilleae, MS Medium, Activated Charcoal, Plant Lets.