Amylase bacteria are enzymes that can hydrolyze starch into sugars. Amylase producing thermophile bacteria can be obtained from hot springs, geothermal, and from compost Compost is a solid mature product obtained from composting process. The search and study of biochemical properties of amylase-producing bacteria originated from have not been done. Compost is rich with various substrates and C/N elements for bacterial growth. This allows the high diversity of amylaseproducing bacteria derived from compost. The aims of this research are to find out and understand the biochemical properties of amylase-producing bacteria derived from compost. The result of bacteria isolation and screening obtained 25 isolates and 5 isolates of amylase-producing bacteria. From 5 isolates of amylase-producing bacteria seen isolate CS3.1 with widest amylolytic index, ie 3.38 mm. CS3.1 bacteria isolates belong to Gram-positive bacteria and have endospores. Based on the results of biochemical properties test showed that bacteria isolate CS3.1 belong to Bacillus sp.

Keywords : bacterial isolation; biochemical test; amylase; compost.