The study was to investigate the role of arbuscular mycorrhiza Fungi spore in soil and tree species create distinct biogeochemical signatures on soils. AM symbioses are usually beneficial to the host as it improves plant survival and performance, extra metrical mycelium the quantification of spores from the soil and extraction techniques. The protocols used for spore extraction are as follows wet-sieve and decant method which was developed by Gerdemann and Nicolson since in the nineteen sixties. Samples were collected from Plate of Mycorrhizal spores that are isolated. Extracting of nematodes serve two purposes and Picking nematodes is easiest with fine forceps and a rubber pipette, under a microscope at low magnification, in a Petri dish as indicated in the case of isolation of Mycorrhizal spores. It is transferred into slide with drop of glycerine and covered with cover slip. Mycorrhizal spores where isolated as shown of the plate