The rapid alarming drug resistance against pathogenic microorganisms is a medical catastrophe for the world. The possible deferring force is to concise the use of antimicrobials synthesised at industrial level and investigate for natural medicinal plant derived compounds having properties of fighting microorganisms. In this regard, the aim of this study was to find the antimicrobial properties of Essential oil obtained from Viola patrinii. The essential oil of Viola patrinii showed lowest inhibition zone about 6.62 mm against S.aureus at the concentration of 250 μg/ml and showed highest zone of inhibition about 16.37 mm against E.coli at the concentration of 1000 μg/ml for bacterial strains. The essential oil of Viola patrinii showed lowest inhibition zone about 8.02 mm against A.niger at the concentration of 250 μg/ml and showed highest zone of inhibition about 15.23 mm against C.albicans at the concentration of 1000 μg/ml for fungal strains. The essential oil Viola patriii showed high antimicrobial activity for both fungal strains and bacterial strains. The order of antimibacterial activity for these strains were E.coli>M.luteus>P.aeruginosa> S.aureus>S.typhi>K.rhizophila>S.epidermis. This investigation lead to the findings that described the antimicrobial potential of this plant essential oil and usefulness of oil against antibiotic resistance. However, further studies are needed to evaluate active compounds and probable medicinal benefits in chemotherapy among humans.

Keywords : Viola Patrinii, Violaceae, Essential Oil, Antibiotic Resistance, Antimicrobials, Inhibition Zone.