In this research work, the bark of Avocado was collected from Pyin Oo Lwin Township, Mandalay Region. The collected sample was tested by phytochemical screening which gives positive for alkaloid, flavonoid, polyphenol, sterol, terpene, glycoside, saponin, and sugar tests. Moreover, the antimicrobial activities of the bark of Avocado was tested by Agar-well diffusion method on six tested organisms such as Bacillus subtilis, Staphylococus aureus, Pseudomonas aeruginosa, Bacillus pumilus, Candida albicans and E-coli respectively. The ethylacetate extract of the bark of Avocado responds high activity on four tested organisms. In addition, organic compound was isolated from the bark of avocado by using thin layer and Column Chromatographic methods. Pure compound (yellow needle like crystal, 0.1755 mg, 0.65% based upon the EtOAc extract) was obtained from the Column Chromatographic technique. The functional groups present in isolated compound were identified by FT-IR spectral data.

Keywords : Avogado, Phytochemical, Antimicrobial, FT- IR, Chromatography.