Biodiesel is an alternative to conventional petroleum diesel. This is a monoalkyl ester from vegetable oil or animal fat. Biodiesel is derived from many oilseeds such as rapeseed, palm oil and castor oil. In this work biodiesel from Moringa Oleifera seed oil is examined. Moringa oleifera oil was extracted on a laboratory scale using a Soxhlet extractor, which was connected to a reflux condenser via a heating jacket at 60C. After acid treatment, the oil is transesterified using methanol, with potassium hydroxide as a catalyst. The synthesized biodiesel is also characterized by flash point, cloud point, pour point, kinematic viscosity and amount of acid from biodiesel, which are 135C, 18C, 17C, 4.85 mm²s‾¹ and 0.26 mg KOH/g respectively. The results are in accordance with ASTM D6751 and EN 14214 biodiesel standards.It was concluded that Moringa oleifera oil has major prospect to be a source for biodiesel production, which can serve as another means of fuel for petro diesel owing to the number of common qualities that they both possess.