- In the present investigations the effect of growth regulators for callus initiation was studied in India’s important Pulse crop Cicer arietinum to observe its response. Six genetic lives of Cicer arietinum were screened for studying morphogenic potential. The intact seedlings were regenerated and develop into shoots from cotyledonary-node on B1 basal medium. However, there was variation in response from different cultivars. Experimental study proved that excellent growth in cytokinin supplemented with benzylaminopurine. The response of regeneration was observed good in combination of cytokinin and benzylaminopurine. When cotyledonary node were isolated, the number of multiple shoots did not increase than on intact seedlings. There was little swelling observed in different explants like roots, hypocotyl and epicotyl.

Keywords : Cicer arietinum, auscius, cytokinin, cotyledon.