Rauwolfia serpentina L Benth Kurz, common name Sarpgandha is a small perennial medicinal shrub of family Apocynaceae. It has a vast array of secondary metabolites, which have a number of commercial applications. The present investigation is an effort to developing an efficient protocol for callus induction using various explants of the same. Callus induction was obtained both from leaf as well as stem explants. It is noteworthy that in the present investigation callus formation from seed explants was also observed in MS basal media (Plate 2). Two different combinations of hormones i.e.2, 4-D + BAP, IAA + BAP, IBA+BAP & Kinetin+NAA with different concentrations were tried for callus induction. In the present research work BAP alone induced callusing in both the explants i.e. leaf and stem, which was 91.54% & 88.83% respectivel

Keywords : Rauwolfia serpentina, medicinal plant, micropropagation, phytohormone, tissue culture.