Background:-Avian influenza is a highly infectious disease caused by type A influenza viruses of the Orthomyxoviridae family, which mainly infect birds. Recently, it was shown to infect all mammalian including humans. This study aimed to determine the serofrequency of a vianflu in poultry farm’s workers in Khartoum State, Sudan.Methods:-This prospective cross-sectional study was conducted in poultry farm workers in Khartoum State, Sudan, during August to September 2016. A total of 60 participants as case group and 30 as control group were enrolled in this study. Blood specimens were collected and serum was obtained. The solid-phase sandwich enzyme-linked immunosorbent assay (ELISA) was used to assay the flu-IgG antibodies in samples. The sociodeogrsphic data and period of their works were gathered by interviewing questionnaire. The obtained data was analyzed by SPSS program.Results:-The avian influenza virus (H5N1) IgG antibodies were detected in 12% (7/60) among case group and 0% (0/30) in control group. The all seropositive result was observed among age group 40-50 years old 100% (7/7). Individuals working at laying hens farm’s had higher serofrequency rate of H5N1 (57%), when compared with those working at meat hens farm’s (43%). Furthermore, the serofrequency of H5NI was higher among slaughtering workers (43%) in comparison with cleaner workers (29%) and (14%) in eggs collector and poultry feeder workers.Conclusion:-The serofrequency of avian flu was frequent in poultry farm workers in Khartoum. Further confirmation with large scale specimen is recommended.

Keywords : Avianflu; H5N1; Serofrequency; Farm workers; IgG; ELISA.