Background and Objectives Chikungunya (CHIK) is a re-emerging disease causing a large negative impact on global health and economics. Clinical manifestations of CHIK are non-specific and difficult to differentiate from dengue hemorrhagic fever or other viral exanthema. A rapid, simple and reliable diagnostic assay is necessary for CHIK outbreak control especially in countries with insufficient access to well-equipped laboratories. This study aimed to detect CHIKV among blood donors in Singa city. Material and Method This is descriptive, cross-sectional study, 90 healthy blood donors who attended Singa Hospital blood bank were included in this study, their age ranged from 20 to 50 years with mean 35 during period from March to November 2017, enzyme linked immunoassay (ELISA) method was used for detection of immunoglobulin antibodies G and M for the CHIK virus from serum samples. Ethical consideration was approved by the research ethical committee of Al Neelain University. Data was collected using direct interviewing questionnaire and analyzed by SPSS. Result out of the total 90 blood donors, serofrequency of CHIK was47(52.2%) and5 (5.5%), for IgG and IgM respectively and 12(13.3%) positive for both. Most of seropositivety (46(51.1%)) was observed among 21-30 year Conclusion seropositivety rate of CHIKV is fairly common among the blood donors in Singa City (Sudan)

Keywords : Chikungunya virus, ELISA, Blood Donors ,Singa.