The infection with chronic hepatitis B (CHB) continues one of the major problem worldwide , which lead to the progressive liver sequelae and the risk of liver failure (LF). So, our study were performed to detect the serum HBe Ag and HBV DNA markers among different stages of CHB patient and their association with biochemical tests levels and predisposing risk factors. A total of one hundred –eight (108) serum samples were collected from CHB patients whom do not treated with any antiviral drugs and were analyzed: Biochemical tests (ALT & AST) , Virological markers (HBs Ag , HBe Ag, anti-HBc IgG) and HBV DNA viral load . An average of CHB patients ages was [(mean ± SD: 38.21±14.16)] years , there were 82 (75.9%) males and 26 (24.1%) were females and all CHB patients were positive for HBs Ag and anti-HBc IgG . Our results showed there were 17(15.7%) of patients were HBe Ag positive and 91(84.3%) were HBeAg negative, HBeAg positive patients with abnormal ALT were 10 (9.3%) , while HBeAg positive patients with abnormal AST were 8 (7.4%). Also, our findings showed there an association were (P < 0.05) between ALT and AST with HBV DNA levels : Patient with high HBV DNA levels with abnormal ALT were 18 (16.7%) , while patients with abnormal AST were 14 (13 %), but, the patients with low HBV DNA levels have abnormal ALT and AST [56 (51.8%) ,8 (7.4 %), respectively]. Interestingly, our data showed that CHB patients with high HBV DNA serum levels and HBeAg positive were 17 (15.7%),while, patients with high HBV DNA levels and HBeAg negative were 19 (17.6%) , but, patients with low HBV DNA serum levels and HBeAg negative 72(66.7%). Finally, our findings revealed that the clinical different stages of CHB patients included : the inactive CHB carrier were 72 (66.7%), the active CHB patients were 19 (17.6%) , the immune tolerant CHB were 11(10.2%), the immune reactive CHB patients were 6 (5.5%) and occult CHB patients were 0 (0%).

Keywords : Chronic hepatitis B, HBe Ag , HBV DNA , CHB stages, Yemen.