Yo-Yo bitters and Evans healthy bitters are brands of polyherbal preparations/mixtures widely consumed in Nigeria. However, there is scarcity of scientific data on the acclaimed medicinal or therapeutic benefits of these bitters. The effects of these bitters on acetaminophen – induced hepatotoxicity in mice was examined using liver function data obtained from the liver of mice. Thirty Wistar mice divided into 6 groups of 5 mice each were used. Group 1 was injected with 0.15 mL of acetaminophen (AAP) and group 4 (control) was injected with 0.03 mL of normal saline. Group 2 and group 3 were injected with 0.3 mL of Yo-Yo bitters and 0.3 mL of Evans Healthy bitters, respectively. Group 5 and 6 were injected with 0.15 mL of AAP and then treated with 0.3 mL of Evans Healthy bitters and 0.3 mL of Yo-Yo bitters, respectively.

Keywords : Evans healthy bitters, Yo-Yo bitters, Acetaminophen, Hepatoprotective, Liver.